1-Step Polymer HISTO-STAT Kits

Background-Free & Wash-Free

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For Wash- Free, Background-Free STAT (rapid) immunostaining in 2 easy steps and under one hour.  Free of avidin, biotin, and streptavidin

A single kit system for animal and human tissue staining

ABOUT POLYMER HISTO-STAT Kits
HISTO-STAT (2-step staining system) is a Non-biotin & Non-streptavidin system developed for wash-free, background-free IHC stainingin 60 minutes. Does NOT require Protein/ serum Blocking.

Innovex Polymer HIsto-Stat kits only involve 2 staining steps post incubation with the primary antibody; an incubation with the Polymer reagent and, a short incubation step (5 minutes) with the substrate/chromogen of choice.
Histo-stat uses one less reagent, one less incubation step and one less rinse step.

UNIQUE FEATURES

No Background. No serum or protein blocking step required
No re-titration of current primary antibodies required, simply use  HISTO-STAT Polymer kits with your current  primary antibody titers
Total staining time of only 60 minutes for paraffin sections and 20-30 minutes for frozen sections 
Wash-Free, only 5 second rinses in between each incubation step.
Free of biotin; No avidin/ biotin blocking required
Short incubation steps
3 years of shelf life
Highly amplified, equates the sensitivity of biotin-streptavidin system

HISTO-STAT USER FRIENDLY PROTOCOL (total staining time: 60 minutes for paraffin section, 20 minutes for frozen sections)

No protein blocking step or extensive rinses are required when staining with Innovex staining systems.

POST DEPARAFFINIZATION

Incubate the section or smear for 20 minutes with primary antibody, rinse once with PBS or Innovex “Signal Enhancing Wash Buffer” for 5 seconds;
Incubate with the Peroxidase (HRP) or alkaline phosphatase Second Step Reagent for 20 minutes, rinse once with PBS or Innovex “Signal Enhancing Wash Buffer” for 5 seconds;
Incubate with INNOVEX substrate/chromogen of choice (AEC or DAB for HRP enzyme label) and Innovex Brown, Fast Red, or other substrate/chromogen available for alkaline phosphatase enzyme for 5-10 minutes, rinse with water;
Counterstain and Mount.

For immunostaining in 60 minutes
POLYMER HISTO-STAT staining systems are available in Complete Staining Systems and in Individual Components

HISTO-STAT, No-wash, No-background Complete Staining Systems include all staining components required including substrate/ chromogen of choice.

HISTO-STAT Complete Staining Systems are available in:
HISTO-STAT PEROXIDASE (HRP) MULTIVALENT COMPLETE SYSTEM (Large Volume)
(single staining system for staining both mouse and rabbit primary antibodies) include :

50 ml of Peroxidase Second Step Reagent for staining both mouse and rabbit primary antibodies
70 ml of stable AEC or DAB of choice.
  Large Volume Stains 600-800 slides

HISTO-STAT PEROXIDASE MULTIVALENT COMPLETE SYSTEM (Small Volume)
(single staining system for staining both mouse and rabbit primary antibodies) include :

20 ml of Peroxidase Second Step Reagent for staining both mouse and rabbit primary antibodies.
30 ml of stable AEC or DAB of choice.
> Small Volume Stains 200-250 slides

HISTO-STAT Alkaline phosphatase Multivalent Complete systems (Large Volume)
(single system for staining both mouse and rabbit primary antibodies) include:

50 ml of alkaline phosphatase Second Step Reagent for staining both mouse and rabbit primary antibodies
Substrate/chromogen of choice, Fast Red (70 ml) or Innovex Brown (30 ml)
> Large Volume Stains 600-800 slides

HISTO-STAT Alkaline phosphatase Multivalent Complete systems (Small Volume)

20ml of alkaline phosphatase Second Step Reagent for staining both mouse and rabbit primary antibodies
Substrate / chromogen of choice, Fast Red (70ml) or Innovex Brown (15ml)
> Small Volume Stains 200-250 slides

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