Universal Animal IHC Kit

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Applicable to all Direct Species (mouse-on-mouse) & all Indirect Species (mouse-on-rabbit)

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STEP 1: UNI-TRIEVE, Water Bath RETRIEVAL
Retrieval Technology using a 60 °C- 70°C Water Bath

  • Gentle and Easy. No Boiling
  • Standardizes Retrieval
  • One solution retrieves for all antibodies and tissues
  • Use a Water Bath
  • Immerse deparaffinized slides into  60-70 °C Uni-Trieve solution for 30 minutes. No Cooling Period required.

STEP 2: BACKGROUND BUSTER
Peptide Blocker for Removal of all Backgrounds including Biotin

  • No avidin block/ serum block or casein block required ( Do not use normal serum or casein containing blockers on animal tissues)
  • Incubate with Background Buster for 30 minutes prior to primary antibody

STEP 3A: STAT-Q KIT (ALL-SPECIES TECHNOLOGY)
Stains all animal tissues/organs including mouse-on-mouse (MOM). Also stains human tissues.

  • 10 min (secondary), 10 min . 5 min.kit
  • Reduces primary antibody incubation to 30 minutes and at the most to 1-hour
  • No more overnight incubation of primary antibodies are required with STAT-Q staining kit

STEP 3B: ENHANCING WASH BUFFER

  • 3-4 fold increase in staining
  • Room temperature amplification minimizes the need for high heat retrieval or enzyme digestion
  • Produces high resolution staining
  • Use in place of PBS or tris buffer for rinse in between incubation steps

Optional Products:
– Fc Receptor Block
May be required in addition to Background Buster when staining CD Markers, Iymphoid tissues, brain  or trans-species Xenograft staining.

REFERENCES