DAB, AEC, & Fast Red Enchancers

Peerless technologies for amplifying IHC stains post completion of staining.

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Innovex Biosciences Chromogen Enhancers are used when stronger staining is desired. They are used to avoid repeating of the entire  staining run and increasing the primary antibody and substrate/ chromogen incubation time to achieve stronger staining.  A short incubation with Chromogen Enhancers amplifies the stain by 2-4 folds and prevents the end user from repeating the staining run and modifying the  primary and secondary reagents titers and incubation times. Chromogen Enhancers produce brilliant and intense staining in a short 5 to 10 minute incubation step. Innovex Chromogen Enhancers amplify staining signals and minimize false negative staining. The use of chromogen Enhancers also allow for reduction of primary antibody Chromogen Enhancers are available in AEC ENHANCER, FAST RED ENHANCER and DAB ENHANCER.


USER FRIENDLY PROTOCOL

  • Incubate with the substrate /chromogen of choice.
  • Rinse with water.
  • Apply the appropriate Innovex Chromogen Enhancer and incubate for 5-10 minutes to intensify staining (the incubation time depends on the chromogen).
  • Rinse with water.
  • Counterstain and mount.

CHROMOGEN ENHANCERS  are best applied before countrstaining, however, THEy MAY BE APPLIED AFTER COUNTERSTAINING.