STAT-Q IHC Staining Kits & kit Components (Background-free)

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From primary antibody to mounted slides under 1-hour

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STAT-Q STAINING KITS FOR QUICK, NO-WASH and NO-BACKGROUND IHC STAINING OF ALL ANIMAL and HUMAN TISSUES, MOUSE-ON-MOUSE, and RABBIT-ON-RABBIT in 1-HOUR

ABOUT STAT-Q
STAT-Q is an amplified Non-polymer technology developed for quick, wash-free, background-free IHC staining of all species tissues and antibodies. STAT-Q stains all human tissues as well as all Animal species tissues including Mouse-on-Mouse, Rabbit-on-Rabbit AND Indirect species such as Mouse-on-Goat, Rabbit-On-Goat; etc and WITHOUT BACKGROUND

AVAILABLE IN DAB AEC Fast Red and permanent Red

UNIQUE FEATURES

  • No serum or protein blocking required for human tissues.
  • No over-nite incubation of primary antibodies. All antibodies stain in 30-minute to 1-hour.
  • Total staining time of only 40 minutes for paraffin sections of human tissues (from primary to mounted slides).
  • Total staining time of 1.5 hours for animal tissues.
  • Total staining time of 15-20 minutes for frozen sections (human tissues).
  • Wash Free, one 5 second rinses in between incubation steps.
  • No Avidin/biotin blocking required.
  • Highly amplified, cuts incubation time of 30 minute primaries to 10 minutes.
  • Allows at least 2 fold increase in dilutions of current primary antibodies.
  • No re-titration of current primary antibody titer is required, simply switch to STAT-Q staining system.
  • Inexpensive.
  • 2 years of shelf life from delivery

STAT-Q USER FRIENDLY PROTOCOL
Total staining time: 35-40 minutes for human and 1.5-hour for animal tissues and mouse-on-mouse
No protein blocking step or extensive rinses are required when staining with Innovex staining systems.

  • Incubate the tissue section with appropriate primary antibody and incubate for 10-30 minutes, rinse once with PBS or Innovex “Signal Enhancing Wash Buffer” for 5 seconds
  • Incubate with STAT-Q secondary linking antibody for 10 minutes, rinse once with PBS or Innovex “Signal Enhancing Wash Buffer” for 5 seconds
  • Incubate with HRP or Alk phos Label for 10 minutes, rinse once with PBS or Innovex Signal Enhancing Wash Buffer” for 5 seconds
  • Incubate with INNOVEX substrate/chromogen of choice (AEC or DAB for HRP enzyme label), Innovex Fast Red or Permanent Red or other substrate/chromogens available for alkaline phosphatase enzyme.
  • Rinse with water, counterstain and mount.

STAT-Q PROTOCOL FOR ANIMAL TISSUES and MOUSE-ON-MOUSE, RABBIT-ON-RABBIT STAINING
For background free animal tissue staining, prior to applying primary antibody incubate all species tissues with Innovex Background Buster (Product # NB306) for 15-minutes for non-identical species such as Mouse-on-Rabbit, Rabbit on Goat. Incubate 30-minutes with Background Buster for identical species such as Mouse-on-Mouse, Rabbit-on-Rabbit and Goat-on Goat. And follow steps 1 to 6 as protocol above.

STAT-Q Kits are available in:
STAT-Q PEROXIDASE (HRP) MULTIVALENT COMPLETE kits (Large Volume kit Stains 800-1500 Slides) Single staining Kit for staining Mouse, Rat and Rabbit primary antibodies includes:

  • 60 ml of STAT-Q amplified Linking antibody for staining mouse, Rat and Rabbit primary antibodies
  • 60 ml of STAT-Q stable and polymerized peroxidase (HRP) labeled streptavidin with no diminishing activity with time
  • 70 ml of stable AEC or DAB of choice.

STAT-Q PEROXIDASE MULTIVALENT COMPLETE KITs (Small Volume Stains 200-400 Slides)

  • 20 ml of STAT-Q amplified Linking antibody for staining Mouse, Rat and Rabbit primary antibodies
  • 20 ml of STAT-Q stable and polymerized peroxidase (HRP) Label with no diminishing activity with time
  • 30 ml of stable AEC or DAB of choice.

STAT-Q ALKALINE PHOSPHATSE COMPLTETE KIT (Large Volume kit Stains 800-1500 Slides)
Single kit for staining Mouse, Rat and Rabbit primary antibodies includes:

  • 60 ml of STAT-Q amplified Linking antibody for staining Mouse, Rat and Rabbit primary antibodies
  • 60 ml of STAT-Q stable and amplified alkaline phosphatase Label
  • Substrate/Chromogen of choice, Innovex FAST RED or Permanent Red

STAT-Q ALKALINE PHOSPHATSE COMPLTETE KIT (Small volume stains 200-400 slides)
Single kit for staining both mouse and rabbit prinary antibodies includes:

  • 20 ml of STAT-Q amplified Linking antibody for staining Mouse, Rat and Rabbit primary antibodies
  • 20 ml of STAT-Q stable alkaline phosphatase Label
  • Substrate/Chromogen of choice, Innovex FAST RED or Permanent Red

STAT-Q Components and Monovalent Secondary Linking antibodies against many animal host species are also available, Click page 2 for details.