1-Step Polymer HISTO-STAT Kits

Background-Free & Wash-Free

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For Wash- Free, Background-Free STAT (rapid) immunostaining in 2 easy steps and under one hour.  Free of avidin, biotin, and streptavidin

A single kit system for animal and human tissue staining

ABOUT POLYMER HISTO-STAT Kits
HISTO-STAT (2-step staining system) is a Non-biotin & Non-streptavidin system developed for wash-free, background-free IHC stainingin 60 minutes. Does NOT require Protein/ serum Blocking.

Innovex Polymer HIsto-Stat kits only involve 2 staining steps post incubation with the primary antibody; an incubation with the Polymer reagent and, a short incubation step (5 minutes) with the substrate/chromogen of choice.
Histo-stat uses one less reagent, one less incubation step and one less rinse step.

UNIQUE FEATURES

  • No Background. No serum or protein blocking step required
  • No re-titration of current primary antibodies required, simply use  HISTO-STAT Polymer kits with your current  primary antibody titers
  • Total staining time of only 60 minutes for paraffin sections and 20-30 minutes for frozen sections 
  • Wash-Free, only 5 second rinses in between each incubation step.
  • Free of biotin; No avidin/ biotin blocking required
  • Short incubation steps
  • 3 years of shelf life
  • Highly amplified, equates the sensitivity of biotin-streptavidin system

HISTO-STAT USER FRIENDLY PROTOCOL (total staining time: 60 minutes for paraffin section, 20 minutes for frozen sections)

No protein blocking step or extensive rinses are required when staining with Innovex staining systems.

POST DEPARAFFINIZATION

  • Incubate the section or smear for 20 minutes with primary antibody, rinse once with PBS or Innovex “Signal Enhancing Wash Buffer” for 5 seconds;
  • Incubate with the Peroxidase (HRP) or alkaline phosphatase Second Step Reagent for 20 minutes, rinse once with PBS or Innovex “Signal Enhancing Wash Buffer” for 5 seconds;
  • Incubate with INNOVEX substrate/chromogen of choice (AEC or DAB for HRP enzyme label) and Innovex Brown, Fast Red, or other substrate/chromogen available for alkaline phosphatase enzyme for 5-10 minutes, rinse with water;
  • Counterstain and Mount.

For immunostaining in 60 minutes
POLYMER HISTO-STAT staining systems are available in Complete Staining Systems and in Individual Components

HISTO-STAT, No-wash, No-background Complete Staining Systems include all staining components required including substrate/ chromogen of choice.

HISTO-STAT Complete Staining Systems are available in:

HISTO-STAT PEROXIDASE (HRP) MULTIVALENT COMPLETE SYSTEM (Large Volume)

(single staining system for staining both mouse and rabbit primary antibodies) include :

  • 50 ml of Peroxidase Second Step Reagent for staining both mouse and rabbit primary antibodies
  • 70 ml of stable AEC or DAB of choice.
      Large Volume Stains 600-800 slides

HISTO-STAT PEROXIDASE MULTIVALENT COMPLETE SYSTEM (Small Volume)
(single staining system for staining both mouse and rabbit primary antibodies) include :

  • 20 ml of Peroxidase Second Step Reagent for staining both mouse and rabbit primary antibodies.
  • 30 ml of stable AEC or DAB of choice.
    > Small Volume Stains 200-250 slides

HISTO-STAT Alkaline phosphatase Multivalent Complete systems (Large Volume)
(single system for staining both mouse and rabbit primary antibodies) include:

  • 50 ml of alkaline phosphatase Second Step Reagent for staining both mouse and rabbit primary antibodies
  • Substrate/chromogen of choice, Fast Red (70 ml) or Innovex Brown (30 ml)
    > Large Volume Stains 600-800 slides

HISTO-STAT Alkaline phosphatase Multivalent Complete systems (Small Volume)

  • 20ml of alkaline phosphatase Second Step Reagent for staining both mouse and rabbit primary antibodies
  • Substrate / chromogen of choice, Fast Red (70ml) or Innovex Brown (15ml)
    > Small Volume Stains 200-250 slides