1-Step Polymer HISTO-STAT Kits
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Background-Free & Wash-Free
Showing all 15 results
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1-Step Polymer HISTO-STAT Peroxidase Multivalent-DAB Kit, stains mouse, rabbit and rat primary antibodies
Large, Product No: NB312LD (600 plus slides)
Small, Product No: NB312LD-20 (200 plus slides)Select options -
1-Step Polymer HISTO-STAT Peroxidase Multivalent AEC Kit, stains mouse, rabbit and rat primary antibodies
Large, Product No: NB312LC (600 plus slides)
Small, Product No: NB312LC-20 (200 plus slides)Select options -
1-Step Polymer HISTO-STAT Alkaline Phosphatase Fast Red Kit, stains mouse, rabbit and rat primary antibodies
Large, Product No: NB317LF (600 plus slides)
Small, Product No: NB317LF-20 (200 plus slides)Select options -
Polymer HISTO-STAT Second step, Alkaline Phosphatase
50 ml, Product No: NB317LB
20 ml, Product No: NB317LB-20Select options -
1-Step Polymer HISTO-STAT Monovalent-Chicken Second Step (HRP) Reagent, stains chicken primary antibodies
15 ml, Product No: NB312C-15
Price: $375.00
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1-Step Polymer HISTO-STAT Monovalent-Goat Second Step (HRP) Reagent, stains goat primary antibodies.
15 ml, Product No: NB312G-15
Price: $375.00
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(US Dollars) -
1-Step Polymer HISTO-STAT Monovalent-Hamster Second Step (HRP) Reagent, stains hamster primary antibodies
15 ml, Product No: NB312H-15
Price: $395.00
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1-Step Polymer HISTO-STAT Monovalent-Mouse Second Step (HRP) Reagent, stains mouse primary antibodies
15 ml, Product No: NB312M-15
Price: $325.00
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(US Dollars) -
1-Step Polymer HISTO-STAT Monovalent-Mouse Second Step Reagent (HRP) Rat-Absorbed, for staining mouse antibodies on rat tissues
15 ml, Product No:
NB312RMA-15Price: $425.00
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1-Step Polymer HISTO-STAT Monovalent-Rabbit Second Step (HRP) Reagent, stains rabbit primary antibodies
15 ml, Product No: NB312R-15
Price: $325.00
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(US Dollars) -
1-Step Polymer HISTO-STAT Monovalent-Rat Second Step (HRP) Reagent, stains rat primary antibodies
15 ml, Product No: NB312T-15
Price: $325.00
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(US Dollars) -
1-Step Polymer HISTO-STAT Monovalent-Rat Second Step Reagent (HRP) Mouse-Adsorbed, for staining rat antibodies on mouse tissues
15 ml, Product No:
NB312MRA-15Price: $425.00
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(US Dollars) -
1-Step Polymer HISTO-STAT Monovalent-Sheep Second Step (HRP) Reagent, stains sheep primary antibodies
15 ml, Product No: NB312S-15
Price: $325.00
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(US Dollars) -
1-Step Polymer HISTO-STAT Multivalent Second Step Reagent (Alkaline phosphatase), stains mouse, rabbit and rat primary antibodies
50 ml, Product No: NB317L
15 ml, Product No: NB317L-15Select options -
1-Step Polymer HISTO-STAT Multivalent Second Step Reagent (HRP), stains mouse, rabbit and rat primary antibodies
50 ml, Product No: NB312L
15 ml, Product No: NB312L-15Select options

For Wash- Free, Background-Free STAT (rapid) immunostaining in 2 easy steps and under one hour. Free of avidin, biotin, and streptavidin
A single kit system for animal and human tissue staining
ABOUT POLYMER HISTO-STAT Kits
HISTO-STAT (2-step staining system) is a Non-biotin & Non-streptavidin system developed for wash-free, background-free IHC stainingin 60 minutes. Does NOT require Protein/ serum Blocking.
Innovex Polymer HIsto-Stat kits only involve 2 staining steps post incubation with the primary antibody; an incubation with the Polymer reagent and, a short incubation step (5 minutes) with the substrate/chromogen of choice.
Histo-stat uses one less reagent, one less incubation step and one less rinse step.
UNIQUE FEATURES
- No Background. No serum or protein blocking step required
- No re-titration of current primary antibodies required, simply use HISTO-STAT Polymer kits with your current primary antibody titers
- Total staining time of only 60 minutes for paraffin sections and 20-30 minutes for frozen sections
- Wash-Free, only 5 second rinses in between each incubation step.
- Free of biotin; No avidin/ biotin blocking required
- Short incubation steps
- 3 years of shelf life
- Highly amplified, equates the sensitivity of biotin-streptavidin system
HISTO-STAT USER FRIENDLY PROTOCOL (total staining time: 60 minutes for paraffin section, 20 minutes for frozen sections)
No protein blocking step or extensive rinses are required when staining with Innovex staining systems.
POST DEPARAFFINIZATION
- Incubate the section or smear for 20 minutes with primary antibody, rinse once with PBS or Innovex “Signal Enhancing Wash Buffer” for 5 seconds;
- Incubate with the Peroxidase (HRP) or alkaline phosphatase Second Step Reagent for 20 minutes, rinse once with PBS or Innovex “Signal Enhancing Wash Buffer” for 5 seconds;
- Incubate with INNOVEX substrate/chromogen of choice (AEC or DAB for HRP enzyme label) and Innovex Brown, Fast Red, or other substrate/chromogen available for alkaline phosphatase enzyme for 5-10 minutes, rinse with water;
- Counterstain and Mount.
For immunostaining in 60 minutes
POLYMER HISTO-STAT staining systems are available in Complete Staining Systems and in Individual Components
HISTO-STAT, No-wash, No-background Complete Staining Systems include all staining components required including substrate/ chromogen of choice.
HISTO-STAT Complete Staining Systems are available in:
HISTO-STAT PEROXIDASE (HRP) MULTIVALENT COMPLETE SYSTEM (Large Volume)
(single staining system for staining both mouse and rabbit primary antibodies) include :
- 50 ml of Peroxidase Second Step Reagent for staining both mouse and rabbit primary antibodies
- 70 ml of stable AEC or DAB of choice.
Large Volume Stains 600-800 slides
HISTO-STAT PEROXIDASE MULTIVALENT COMPLETE SYSTEM (Small Volume)
(single staining system for staining both mouse and rabbit primary antibodies) include :
- 20 ml of Peroxidase Second Step Reagent for staining both mouse and rabbit primary antibodies.
- 30 ml of stable AEC or DAB of choice.
> Small Volume Stains 200-250 slides
HISTO-STAT Alkaline phosphatase Multivalent Complete systems (Large Volume)
(single system for staining both mouse and rabbit primary antibodies) include:
- 50 ml of alkaline phosphatase Second Step Reagent for staining both mouse and rabbit primary antibodies
- Substrate/chromogen of choice, Fast Red (70 ml) or Innovex Brown (30 ml)
> Large Volume Stains 600-800 slides
HISTO-STAT Alkaline phosphatase Multivalent Complete systems (Small Volume)
- 20ml of alkaline phosphatase Second Step Reagent for staining both mouse and rabbit primary antibodies
- Substrate / chromogen of choice, Fast Red (70ml) or Innovex Brown (15ml)
> Small Volume Stains 200-250 slides