IHC Enhancing Wash Buffers

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Advanced Features:

Simple to use, employ as the rinse buffer in place of PBS for rinsing in between incubation steps
Amplify staining signal by 2-4 folds with no additional steps added to the immunostaining procedure
Cost effective: Allows 2-4 folds increase for primary antibody dilution
Time Saver : Allows for decrease in primary antibody incubation time
Allows for decrease in chromogen/substrate incubation time
Fixation independent; Amplifies staining regardless of the type of fixative employed
Eliminates enzyme pre-digestion
Minimizes the need for high heat antigen recovery when used with INNOVEX StainingSystems
Minimizes false negative staining
Automation compatible
Applicable to ELISA and flow cytometric procedures

AEC staining of UCHL-1 (T cell) antibody of bone marrow, paraffin section rinsed with PBS

Same section amplified when simply rinsed with Innovex Enhancing Wash Buffer— No heat treatment

User-Friendly Protocol

Innovex Enhancing Wash Buffers only requires seconds rinse in between incubation steps when used with Innovex STAT-Q and HISTO-STAT( 2-step) staining systems. Innovex Enhancing Wash Buffers may be employed with other systems, observe the manufacturer’s instructions for the required time and number of washes recommended for the system.

Rinse specimens with Innovex Enhancing Wash Buffer prior to applying primary anti-body
Incubate with primary antibody per manufacturer’s instructions
Rinse with Innovex Enhancing Wash Buffer
Incubate with secondary antibody
Rinse with Innovex Enhancing Wash Buffer
Incubate with Enzyme label
Rinse with Innovex Enhancing Wash Buffer
Incubate with substrate/chromogen of choice
Rinse with water
Counterstain and mount.

For ELISA assays
Innovex Enhancing Wash Buffers can be used for washing ELISA plates, however, some non-ionic detergent should be added to the solution.

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