1-Step Polymer HISTO-STAT Kits
Background-Free & Wash-Free
Showing all 15 results
-
Large, Product No: NB312LD (600 plus slides)
Small, Product No: NB312LD-20 (200 plus slides) Select options1-Step Polymer HISTO-STAT Kits1-Step Polymer HISTO-STAT Peroxidase Multivalent-DAB Kit, stains mouse, rabbit and rat primary antibodies
$655.00 – $895.00 -
Large, Product No: NB312LC (600 plus slides)
Small, Product No: NB312LC-20 (200 plus slides) Select options1-Step Polymer HISTO-STAT Kits1-Step Polymer HISTO-STAT Peroxidase Multivalent AEC Kit, stains mouse, rabbit and rat primary antibodies
$655.00 – $895.00 -
Large, Product No: NB317LF (600 plus slides)
Small, Product No: NB317LF-20 (200 plus slides) Select options1-Step Polymer HISTO-STAT Kits1-Step Polymer HISTO-STAT Alkaline Phosphatase Fast Red Kit, stains mouse, rabbit and rat primary antibodies
$655.00 – $955.00 -
1-Step Polymer HISTO-STAT Kits
Polymer HISTO-STAT Second step, Alkaline Phosphatase
$495.00 – $785.00 -
1-Step Polymer HISTO-STAT Kits
1-Step Polymer HISTO-STAT Monovalent-Chicken Second Step (HRP) Reagent, stains chicken primary antibodies, Product No: NB312C-15
$390.00 -
1-Step Polymer HISTO-STAT Kits
1-Step Polymer HISTO-STAT Monovalent-Goat Second Step (HRP) Reagent, stains goat primary antibodies, Product No: NB312G-15
$390.00 -
1-Step Polymer HISTO-STAT Kits
1-Step Polymer HISTO-STAT Monovalent-Hamster Second Step (HRP) Reagent, stains hamster primary antibodies, Product No: NB312H-15
$395.00 -
1-Step Polymer HISTO-STAT Kits
1-Step Polymer HISTO-STAT Monovalent-Mouse Second Step (HRP) Reagent, stains mouse primary antibodies, Product No: NB312M-15
$345.00 -
1-Step Polymer HISTO-STAT Kits
1-Step Polymer HISTO-STAT Monovalent-Mouse Second Step Reagent (HRP) Rat-Absorbed, for staining mouse antibodies on rat tissues, Product No: NB312RMA-15
$445.00 -
1-Step Polymer HISTO-STAT Kits
1-Step Polymer HISTO-STAT Monovalent-Rabbit Second Step (HRP) Reagent, stains rabbit primary antibodies, Product No: NB312R-15
$345.00 -
1-Step Polymer HISTO-STAT Kits
1-Step Polymer HISTO-STAT Monovalent-Rat Second Step (HRP) Reagent, stains rat primary antibodies, Product No: NB312T-15
$345.00 -
1-Step Polymer HISTO-STAT Kits
1-Step Polymer HISTO-STAT Monovalent-Rat Second Step Reagent (HRP) Mouse-Adsorbed, for staining rat antibodies on mouse tissues, Product No: NB312MRA-15
$445.00 -
1-Step Polymer HISTO-STAT Kits
1-Step Polymer HISTO-STAT Monovalent-Sheep Second Step (HRP) Reagent, stains sheep primary antibodies, Product No: NB312S-15
$345.00 -
1-Step Polymer HISTO-STAT Kits
1-Step Polymer HISTO-STAT Multivalent Second Step Reagent (Alkaline phosphatase), stains mouse, rabbit and rat primary antibodies
$395.00 – $590.00 -
1-Step Polymer HISTO-STAT Kits
1-Step Polymer HISTO-STAT Multivalent Second Step Reagent (HRP), stains mouse, rabbit and rat primary antibodies
$395.00 – $495.00
For Wash- Free, Background-Free STAT (rapid) immunostaining in 2 easy steps and under one hour. Free of avidin, biotin, and streptavidin
A single kit system for animal and human tissue staining
ABOUT POLYMER HISTO-STAT Kits
HISTO-STAT (2-step staining system) is a Non-biotin & Non-streptavidin system developed for wash-free, background-free IHC stainingin 60 minutes. Does NOT require Protein/ serum Blocking.
Innovex Polymer HIsto-Stat kits only involve 2 staining steps post incubation with the primary antibody; an incubation with the Polymer reagent and, a short incubation step (5 minutes) with the substrate/chromogen of choice.
Histo-stat uses one less reagent, one less incubation step and one less rinse step.
UNIQUE FEATURES
No Background. No serum or protein blocking step required
No re-titration of current primary antibodies required, simply use HISTO-STAT Polymer kits with your current primary antibody titers
Total staining time of only 60 minutes for paraffin sections and 20-30 minutes for frozen sections
Wash-Free, only 5 second rinses in between each incubation step.
Free of biotin; No avidin/ biotin blocking required
Short incubation steps
3 years of shelf life
Highly amplified, equates the sensitivity of biotin-streptavidin system
HISTO-STAT USER FRIENDLY PROTOCOL (total staining time: 60 minutes for paraffin section, 20 minutes for frozen sections)
No protein blocking step or extensive rinses are required when staining with Innovex staining systems.
POST DEPARAFFINIZATION
Incubate the section or smear for 20 minutes with primary antibody, rinse once with PBS or Innovex “Signal Enhancing Wash Buffer” for 5 seconds;
Incubate with the Peroxidase (HRP) or alkaline phosphatase Second Step Reagent for 20 minutes, rinse once with PBS or Innovex “Signal Enhancing Wash Buffer” for 5 seconds;
Incubate with INNOVEX substrate/chromogen of choice (AEC or DAB for HRP enzyme label) and Innovex Brown, Fast Red, or other substrate/chromogen available for alkaline phosphatase enzyme for 5-10 minutes, rinse with water;
Counterstain and Mount.
For immunostaining in 60 minutes
POLYMER HISTO-STAT staining systems are available in Complete Staining Systems and in Individual Components
HISTO-STAT, No-wash, No-background Complete Staining Systems include all staining components required including substrate/ chromogen of choice.
HISTO-STAT Complete Staining Systems are available in:
HISTO-STAT PEROXIDASE (HRP) MULTIVALENT COMPLETE SYSTEM (Large Volume)
(single staining system for staining both mouse and rabbit primary antibodies) include :
50 ml of Peroxidase Second Step Reagent for staining both mouse and rabbit primary antibodies
70 ml of stable AEC or DAB of choice.
Large Volume Stains 600-800 slides
HISTO-STAT PEROXIDASE MULTIVALENT COMPLETE SYSTEM (Small Volume)
(single staining system for staining both mouse and rabbit primary antibodies) include :
20 ml of Peroxidase Second Step Reagent for staining both mouse and rabbit primary antibodies.
30 ml of stable AEC or DAB of choice.
> Small Volume Stains 200-250 slides
HISTO-STAT Alkaline phosphatase Multivalent Complete systems (Large Volume)
(single system for staining both mouse and rabbit primary antibodies) include:
50 ml of alkaline phosphatase Second Step Reagent for staining both mouse and rabbit primary antibodies
Substrate/chromogen of choice, Fast Red (70 ml) or Innovex Brown (30 ml)
> Large Volume Stains 600-800 slides
HISTO-STAT Alkaline phosphatase Multivalent Complete systems (Small Volume)
20ml of alkaline phosphatase Second Step Reagent for staining both mouse and rabbit primary antibodies
Substrate / chromogen of choice, Fast Red (70ml) or Innovex Brown (15ml)
> Small Volume Stains 200-250 slides